Extended Data Fig. 5: Comparison of cell isolation methods and reference datasets.
a,b, Additional scRNAseq data was generated as described, except that BM was derived by flushing bones and subjected or not subjected to enzymatic digestion. Data was projected to two dimensions using t-SNE and cell type labels were assigned using the anchoring approach implemented in seurat353. N=2411 cells are shown. c,d, Single-cell RNA-seq data from a recent study of different genetically labelled populations from flushed BM26 was projected to two dimensions using t-SNE and cell type labels were assigned using the anchoring approach implemented in seurat353. N=6617 cells are shown. e, Comparison of cell type frequencies between various published datasets. For Tikhonova et al.26, n=6617 cells, Baryawno et al.25, n=25933 cells, Baccin et al., n=7497 cells.
