Extended Data Fig. 7: Kinase SYK phosphorylates BCAT2 at Y228 site.
From: BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma
a, Identification of SYK as tyrosine kinase for BCAT2 phosphorylation at 228 tyrosine residue (Y228) using tyrosine kinase library screening. b, Y228F mutant decreases SYK-mediated phosphorylation of BCAT2. c, Enhanced ubiquitylation of BCAT2WT, but not BCAT2Y228 mutant by SYK in HEK293T cells. d, Representative images SYK- and BCAT2-staining of serial sections from adjacent tissues and PDAC specimens (n = 22 biologically independent patients, acinar, adjacent duct, PDAC area and stroma cells were indicated as asterisk, arrowhead, arrow and dotted arrow, respectively. Scale bar: 12.5 μm). Data are representative of three biologically independent experiments in a, b and c.
