Extended Data Fig. 9: Low-BCAA diet impedes PDAC development in KC mice.
From: BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma
a, Decreased plasma BCAA concentration in mice fed on low-BCAA diet (mean ± s.e.m. of n = 8 biologically independent animals, two-tailed t-test). b,c, Significantly decreased pancreas weight of mice at the age of 4 (b, mean ± s.e.m. of n = 7 biologically independent animals, two-tailed t-test) and 6 (c, mean ± s.e.m. of n = 6 biologically independent animals, two-tailed t-test) months by feeding with low-BCAA diet. d-f, Impeded PanIN lesions of KC mice at the age of 2, 4, and 6 months by low-BCAA diet. Representative images (d, data are representative of three biologically independent experiments) and quantification of alcian blue (e, each 7 fields, mean ± s.e.m., two-tailed t-test)- and sirius red (f, each 7 fields, mean ± s.e.m., two-tailed t-test)-staining (scale bars: 50 μm). g-h, Suppression of pancreatic duct cell growth in PanIN lesions of KC mice by low-BCAA diet. Representative images (g, data are representative of three biologically independent experiments) and quantification of Ki67 (h, each 10 fields, mean ± s.e.m., two-tailed t-test)- and CK19-staining (scale bars: 12.5 μm). i, No significant change of food intake, of Control (Con) and KC mice fed with normal or low-BCAA diet (mean ± s.e.m. of n = 8 biologically independent samples, two-tailed t-test). j, Low-BCAA diet results in decreased body weight in both Con and KC mice (mean ± s.e.m. of n = 8 biologically independent animals, ***p < 0.001, detailed p value is available in Supplementary Table 3). k, l, No significant effect on glucose homeostasis of KC mice by low-BCAA diet. GTT and ITT were analyzed (mean ± s.e.m. of n = 4 biologically independent animals (k), n = 5 biologically independent animals (l), n.s. donates for no significance, detailed p value is available in Supplementary Table 3, two-tailed t-test). m, Low-BCAA diet decreases density of skeletal muscle. Representative images of H&E staining for different organs harvested at the age of 4 month (n = 5 biologically independent animals per group, scale bars: 50 μm).
