Extended Data Fig. 1: BCAT2 but not BCAT1 is elevated in PDAC cells.
From: BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma
a, Alteration in amino acids concentrations in pancreatic tissue of KC mice at the age of 2 months (mean ± s.e.m. of n = 6 biologically independent animals, two-tailed t-test). b, Increased BCAT2 protein level in PDAC cells, by comparing to slight difference of BCAT1 expression (data are representative of three biologically independent experiments). c-e, Representative images of Bcat1 and Bcat2 staining of pancreatic tissue specimens from Control (Con) (n = 20 biologically independent animals), KC (n = 20 biologically independent animals), and KPC (n = 10) mice and human PDAC (n = 72 biologically independent patients). f. Representative images and quantification of Pi-Bckdha, Bckdha, Hmgcl, Acadm, Hadh in KC mice (mean ± s.e.m. of n = 6 biologically independent animals, median indicated as dotted lines, two-tailed t-test). Pancreatic acinar, islet, normal pancreatic duct, PanIN duct, and PDAC area were indicated as asterisk, rhombus, arrowhead, arrow, and dotted arrow, respectively. Scale bar: c, d, e: 12.5 μm; f: 50 μm.
