Extended Data Fig. 2: Determination of FERARI subunits interactions.
From: FERARI is required for Rab11-dependent endocytic recycling
a, Pull-downs showing interaction between HA-RABS-5 and fragments of UNC-44 (coupled to FLAG). UNC-44Z was not expressed, anti-FLAG antibody cross-reacted with RABS-5 (indicated by asterisk). n=3 independent experiments. Quantification is shown in (a’); mean ± s. d. is given. b, Western blot of the interactions between transiently over-expressed human FERARI components rabenosyn-5, VIPAS39 and VPS45. GFP-Rabenosyn-5 was precipitated with GFP-trap beads, and myc-VIPAS39 and myc-VPS45 co-precipitated. n= 3 independent experiments. Quantification is shown in (b’); mean ± s. d. is given. c, Pull-down experiment showing the interaction of SNX-1 with SPE-39 and RABS-5. n=3 independent experiments. Quantification is shown in (c’); mean ± s. d. is given. d, Interactions between SPE-39 and VPS-45 as well as VPS-45 and RABS-5 in pull-down experiments. n=3 independent experiments. Quantification is shown in (d’); mean ± s. d. is given. See Unprocessed Blots Extended Data 2 and Statistical Source Data Extended Data 2.
