Extended Data Fig. 3: Progressive loss of progenitor cells in Sel1l-knockout mice.
a, Western blot analysis of SEL1L expression in LSK cells from 2 control (Ctrl) and 2 Sel1lMx1 mice 1 week after last poly (I:C) injection. β-ACTIN was used as loading control. b, Bone marrow (2 femurs and 2 tibiae) cellularity of Ctrl and Sel1lMx1 mice at the indicated time points. -1 indicates 1 week before poly (I:C) injection. Age and gender matched littermates were used in each time points. Ctrl (12, 20): n = 5, Ctrl (5): n = 6 and the others: n = 4. c, d, Percentage (c) and number (d) of CMP, GMP, MEP and CLP in the BM of control (Ctrl) and Sel1lMx1 mice at the indicated time points. Ctrl (12, 20): n = 5, Ctrl (5): n = 6 and the others: n = 4. e, f, Percentage (e) and number (f) of B, T and myeloid cells in the BM of control (Ctrl) and Sel1lMx1 mice at the indicated time points. Ctrl and Sel1lMx1 (1, 12): n = 3, Ctrl (5): n = 6 and the others: n = 4. g, Representative image of femur bone from control and Sel1lMx1 mice 30 weeks after poly (I:C) injection. h, i, Quantification of Red blood cell (RBC, h) and haemoglobin (HGB, i) in peripheral blood of Ctrl and Sel1lMx1 mice 30 weeks after poly (I:C) injection. n = 4. j-l, Body weight (j), spleen weight (k) and thymus weight (l) in control and Sel1lMx1 mice as in h. n = 4. m, n, BM (m) and spleen (n) cellularity in mice as in h. n = 4. Results are shown as mean ± s.d. Two-way ANOVA (b-f) or two-tailed Student’s t-tests (h-n) was used to assess statistical significance. ns, not significant. Statistical information and unprocessed blots are provided as source data.
