Extended Data Fig. 7: Effect of perturbations to mitochondrial dynamics on MRG distribution.
From: Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics
Representative widefield-microscopy images of HeLa cells silenced using siRNAs against Drp1 (second row) or Mfn2 (third row). Cells were fixed after 72 hours of silencing. A negative control siRNA was used in parallel, and is shown in the first row. MRGs and nucleoids were immunolabeled using anti-FASTKD2 (green) and anti-DNA (magenta), respectively. Mitochondria were labelled using MitoTracker Deep Red staining (cyan) (The experiment has been performed twice with similar results). Scale-bar: 10 µm. Disrupted MRG and nucleoid positioning, and clumped appearance as described for mito-bulbs is apparent when either Drp1 and Mfn2 are silenced, but the negative control shows well-dispersed MRGs and nucleoids.
