Extended Data Fig. 4: MRG and nucleoid dynamics in live cells.
From: Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics
a, Representative time-lapse images of an MRG fusion event in a live U2OS cell, monitored by SIM. MRGs are visualized by stable expression of FASTKD2-eGFP (n= 7 cells). b, Representative time-lapse images of an MRG splitting event in a live COS-7 cell, monitored by SIM. MRGs are visualized by stable expression of FASTKD2-eGFP (n= 6 cells). c, d, Representative time-lapse images of nucleoid “kiss-and-run” (n=1 cell) and splitting events (n=2 cells), respectively, in COS-7 cells, monitored by SIM. Nucleoids are visualized by transient expression of TWINKLE-eGFP and mitochondrial outlines are highlighted by TOMM20-eGFP expression. a–d, Cells were imaged at 1/5 Hz. White arrowheads indicate the dynamic events. Dashed lines indicate the segments used to measure the intensity (grey values) represented in the plots below. Linear Fire LUTs are used to represent pixel intensity values. Scale bars: 2 μm. Statistical source data are provided in Source data Extended data Fig. 4.
