Extended Data Fig. 5: Characterization of the intracellular localization of LRRC31 with Ku70 and ATR as well as the effects of LRRC31 on DNA–PKcs recruitment.
From: LRRC31 inhibits DNA repair and sensitizes breast cancer brain metastasis to radiation therapy
a, b, Confocal analysis of the intracellular localization of LRRC31 and Ku70 in 231BR cells without (a) and with (b) overexpression of LRRC31. Irradiation was performed at 4 Gy. Scale bar: 10 µm. c, d, IP-WB analysis (c) and semi-quantification (d) of the effect of LRRC31 downregulation or overexpression on DNA–PKcs recruitment in 231BR cells. e, f, Confocal analysis of the intracellular localization of LRRC31 and ATR in 231BR cells without (e) and with (f) overexpression of LRRC31. Irradiation was performed at 4 Gy. Scale bar: 10 µm. g, h, IP-WB analysis (g) and semi-quantification (h) of the effect of LRRC31 downregulation or overexpression on DNA–PKcs recruitment in MCF7 cells. For all the studies, three biologically independent experiments were performed with similar results obtained. Data in c and f show the mean ± s.d. (n = 3 biologically independent experiments). Unprocessed immunoblots are shown in Source Data Extended Data Fig. 5.
