Extended Data Fig. 7: Characterization of LRRC31 as a therapeutic target.
From: LRRC31 inhibits DNA repair and sensitizes breast cancer brain metastasis to radiation therapy
Characterization of LRRC31 as a therapeutic target. a, Analysis of the expression of LRRC31 in the indicated tumors in the TCGA database using Gene Expression Profiling Interactive Analysis (GEPIA). BRCA: breast invasive carcinoma; COAD: colon adenocarcinoma; LIHC: liver hepatocellular carcinoma; LUAD: lung adenocarcinoma; PAAD: pancreatic adenocarcinoma; PRAD: prostate adenocarcinoma; READ: rectum adenocarcinoma. b, Analysis of the correlation of patient survival with LRRC31 expression in BRCA patients using OncoLnc. Analysis was performed by comparing those patients with the expression level of LRRC31 among top 80th percentile (high, n = 805 biologically independent samples) with the rest (low, n = 200 biologically independent samples). c, Analysis of the correlation of patient survival with LRRC31 expression in READ patients. Analysis was performed by comparing those patients with the expression level of LRRC31 among top 80th percentile (high, n = 127 biologically independent samples) with the rest (low, n = 31 biologically independent samples). Statistical analyses for b and c were performed using the Log-rank (Mantel-Cox) test. d, Schematic diagram of characterization of LRRC31 NP-mediated gene therapy in mice bearing intracranial 231BR tumors. Cells were engineered to express both luciferase and GFP. e, f, Representative images of tumors in the brain imaged by IVIS (e) and semi-quantification of the bioluminescence signal (f) in tumor-bearing mice received intravenous administration of the indicated NPs following with and without irradiation treatment (5 Gy×2). Data in f show the mean ± s.d. (n = 5 animals). Statistical analysis was performed using the two-tailed, unpaired Student’s t-test. g, Ex vivo imaging the brains isolated from the mice received the indicated treatment. h-j, Representative images of H&E (h), Caspase-3(i), and Ki67 (j), staining of tumors isolated from mice received the indicated treatment. Scale bar: 100 µm. Three biologically independent experiments were performed with similar results obtained.
