Extended Data Fig. 2: EMT hallmarks are upregulated in OE hearts.
From: ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration
a, Analysis of bulk RNA- seq of adult WT–MI (n=4) and OE–MI (n=4) hearts using GSEA hallmark module (relates to Fig. 1h). Bar plot depict the normalized enrichment scores (NES). b-e, RNA- seq derived heat map of the indicated genes belonging to the EMT hallmark (as shown in (a) and Fig. 1h). Genes were annotated as (b) ECM composition (c) ECM modulators (d) Transcription factors and (e) Transmembranal and secreted proteins. FC to the right indicates OE/WT fold change of the detected transcripts by RNA- seq using a threshold FC≥1.5 and p adjusted ≤0.05. Statistics were determined by DESeq2, and adjusted for multiple testing using the procedure of Benjamini and Hochberg. f, Representative image in adult WT and OE hearts. Scale bar, 50 µm.
