Extended Data Fig. 1: Apoptotic clusters are randomly distributed throughout fetal testes.
From: Apoptosis in the fetal testis eliminates developmentally defective germ cell clones
a, Percent apoptotic cleaved-PARP+ germ cells in whole-mount testes on a mixed genetic background during the apoptotic wave. n=4 animals. Data presented as mean values ± SD. Dashed line denotes reported average apoptotic index in developing fetal tissues18. b, Percent cleaved-PARP+ cells on apoptosis-sensitive C57/Bl6 background20 at peak apoptosis (E13.5). c, Distribution of apoptotic clusters measured for spatial bias. 3D coordinates of each cluster’s centroid were evaluated by Ripley’s K function. These were compared against simulated distributions for an equivalent number of points. Representative plots depict each simulated distribution type (right). Cluster coordinates from n=5 animals.**: p=0, †: p=0.10622, between-treatments sum of squares. d, E13.5 testis section stained for Sertoli cells (AMH), germ cells (TRA98), and apoptosis (cleaved-PARP). Apoptotic cords are defined as cords containing any apoptotic germ cells (red outline) in contrast to non-apoptotic cords (white outline). Scale bar = 20µm. e, Ratio of total germ cells to Sertoli cells in apoptotic and non-apoptotic cords, p=0.584, two-tailed Student’s t-test. Data points represent individual cords pooled from sections from 4 animals. n =15 non-apoptotic cords and n = 11 apoptotic cords. Data presented as mean values ± SD. f, Average numbers of Sertoli cells in direct contact with each germ cell in apoptotic and non-apoptotic cords. Within apoptotic cords, apoptotic (PARP+) and non-apoptotic (PARP-) germ cells were separately considered. p=0.737154, one-way ANOVA. Data points represent individual germ cells (n) analysed in sections from 4 animals. Total cell counts per category are detailed in source data. Data presented as mean values ± SD. g, Quantification of apoptotic germ cells in E13.5 Tex14 mutants. p=0.4491, two-tailed Student’s t-test. n=4 animals per genotype. Data presented as mean values ± SD. h, Apoptotic cluster size by cell number in Tex14 E13.5. p=0.0073, two-tailed Student’s t-test. Data points (n) are available in source data and represent individual apoptotic clusters analysed from 2 animals (Tex14+/+) and 3 animals (Tex14-/-). Data presented as mean values ± SD.
