Extended Data Fig. 9: Full panel of chimeric E12.5 placentas generated using tetraploid complementation.
From: Evaluating totipotency using criteria of increasing stringency
Immunofluorescent staining of E12.5 chimeric placentas generated with H2B-GFP expressing tetraploid host embryo and wild-type ESCs (left panel), wild-type tetraploid host embryo and H2B-GFP expressing ESCs (middle panel) and wild-type host embryo (diploid) and H2B-GFP expressing L-EPSCs. Placenta sections are immuno-stained for GFP and the following trophoblast markers: CDH3, TPBPA, and MCT4. A total of 14 H2B-GFP positive L-EPSCs placentas were collected from two females, and 3 to 4 placentas were analyzed for each marker. Images shown at different magnifications. Scale bars: 500 µ (4x), 200 µ (7x), 50 µ (40x). Sections from 4 chimeric placentas were analyzed.
