Extended Data Fig. 4: Increasing Asn promotes T cell activity in vivo.

a-j, C57BL/6 mice intraperitoneally injected with Asn or PBS (Ctrl) every two days for a period lasting one month. Spleens were excised, weighted and pictured (a). Relative numbers of cells in spleens and lymph nodes (b), weight of mice (c), Percentage of CD4⁺ T cells and CD8⁺ T cells relative to total cells in spleens and lymph nodes (d, e), effector (e) and central memory-like (f) CD8⁺ T cells relative to total CD8⁺ T cells in spleens and lymph nodes, CD25⁺CD4⁺ T cells (h), and effector (i) and central memory-like (j) CD4⁺ T cells relative to total CD4⁺ T cells in spleens and lymph nodes were assessed. k-r, C57BL/6 mice intraperitoneally injected with ASNase or PBS (Ctrl) every two days for a period lasting one month. Spleens were excised, weighted and pictured (k). Relative numbers of cells in spleens and lymph nodes (l), percentage of CD4⁺ T cells and CD8⁺ T cells relative to total cells in spleens and lymph nodes (m), effector memory-like (n) and central memory-like (o) CD8⁺ T cells relative to total CD8⁺ T cells in spleens and lymph nodes, percentage of CD25⁺CD4⁺ T cells (p), effector memory-like (q) and central memory-like (r) CD4⁺ T cells relative to total CD4⁺ T cells in spleens and lymph nodes were assessed. s, Surface expression of CD25 and CD44 on mouse naive CD8⁺ T cells activated with serum from normal diet mice intraperitoneally injected with PBS (Ctrl) or ASNase (n = 6 mice per group). All data are mean ± SEM, in a-r, Ctrl, n = 7 mice; Asn, n = 6 mice. Two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant. Related representative FACS plots (Supplementary Figs. 13, 14) and numerical source data for a-s are provided.

Source data