Extended Data Fig. 3: Extended analysis of PARPi sensitivity upon ALC1 loss.
a, Sensitivities of the indicated DLD1 BRCA2-/- cells to vel (veliparib), ola and tal in CellTiter-Glo assay. Data are mean ± s.e.m. from n = 3 biologically independent experiments. b, Immunoblot showing ALC1 levels in cells used for xenograft studies (first four left lanes) and in tumors that reached >10.5 mm in any dimension, which is when the mice were euthanized. Data from two biologically independent tumors per condition are shown. c, GFP competition experiment in UWB1.289+BRCA1 addback line to examine the effects of the combined loss of ALC1 and the indicated DNA repair proteins on cell proliferation and ola sensitivity. Data are normalized to Tinitial and indicate mean ± s.e.m. After every two population doublings, cells were passaged (P) and GFP percent was recorded (n=4 independent transductions except for sgFEN1, where n = 6 independent transductions were performed). Source data are provided.
