Extended Data Fig. 1: ALC1 loss renders olaparib hypersensitivity and proliferation defects in various BRCA-mutant lines. | Nature Cell Biology

Extended Data Fig. 1: ALC1 loss renders olaparib hypersensitivity and proliferation defects in various BRCA-mutant lines.

From: ALC1 links chromatin accessibility to PARP inhibitor response in homologous recombination-deficient cells

Extended Data Fig. 1: ALC1 loss renders olaparib hypersensitivity and proliferation defects in various BRCA-mutant lines.

a, Protein domains ranked on the basis of the CRISPR score (CS) for ola sensitivity in BRCA1-mutant UWB1.289 cells. b, Immunoblot showing depletion of ALC1 using a sgRNA vector with GFP selection marker. GFP-positive cells were sorted and analyzed. The blot is a representative image of two biologically independent experiments. c, GFP competition assay to examine the effects of ALC1 depletion on ola sensitivity in CAPAN-1, SUM149PT and UWB1.289 cells. Ola sensitivity in CAPAN-1 and SUM149PT was confirmed using six and seven independent guides respectively and data for each guide are from one experiment performed at three different ola concentrations. Ola sensitivity in UWB1.289 was confirmed using seven independent guides and data for each guide is mean of two biologically independent experiments in the absence and presence of 50 nM ola concentration. Source data are provided.

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