Extended Data Fig. 3: Manipulation of Rheb activity affects OPP incorporation in wild type and RPS23R67K/ + imaginal discs.
From: Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition
(a–d) Wing imaginal discs (wild type and RPS23R67K/+, as indicated) overexpressing Rheb or RhebRNAi in the anterior compartment (left hand side of the disc). The discs were explanted and incubated for a 15 min in 1 µM OPP before staining for puromycilated peptides (grey scale). Rheb overexpression stimulated OPP incorporation in both genotypes, while RhebRNAi had the opposite effect. Scale bars represent 50 µm. Genotypes for each figure panel are available in Supplementary Table 1. Experiments were repeated independently three times with similar results.
