Extended Data Fig. 3: Autophagy flux in ribosome mutants and upon translation inhibition.
From: Proteotoxic stress is a driver of the loser status and cell competition
(a-c) GFP-p62 ReFlux signal (green) in wing discs expressing RNAi against the autophagy gene atg1 specifically in P cells (labelled by the absence of Ci, magenta), immediately after heat shock (a) or three hours later (b), and corresponding signal quantifications (n = 7 and 6 respectively, two-sided two sample Kolmgorov-Smirnov test) (c). (d-f) GFP-p62 ReFlux signal (green) in a wing disc harboring RpS3+/− clones (dsRed-positive) three hours after heat-shock (d) and corresponding quantification of GFP-p62 signal intensity (e) and number of GFP-p62 foci per area (f) (for both measurements, n = 5, two-sided paired t-test). (g) GFP-p62 ReFlux signal (green) in wing discs harboring RpS3+/− A cells and wild-type P cells, three hours after heat-shock, with or without addition of chloroquine, as indicated. (h) GFP-p62 ReFlux signal (green) in wing discs harboring RpS3+/− A cells (dsRed-positive) and wild-type P cells (dsRed-negative) twenty-four hours after heat-shock. (i-k) GFP-p62 ReFlux signal (green) in wing discs harboring wild-type A cells and 4E-BPTA-expressing P cells (labelled by the absence of Ci, magenta), immediately after heat shock (i) or three hours later (j), and corresponding signal quantifications relative to wing discs containing an RpS3+/− A compartment and wildtype P compartment (images not shown) (n = 9 and 8 for 0 and 3 hour 4E-BPTA, and n = 7 and 8 for 0 and 3 hour RpS3+/−, respectively; two-sided two-sample Kolmgorov-Smirnov test without p-adjustment for multiple comparisons) (k). For all micrographs, scale bars correspond to 50 µm. For all quantifications provided, the horizontal line represents the mean and whiskers indicate 95% confidence intervals. All n numbers refer to the number of individual wing discs.
