Extended Data Fig. 1: RYBP-PRC1 is recruited to chromatin by the direct interaction between H2AK119ub1 and RYBP. | Nature Cell Biology

Extended Data Fig. 1: RYBP-PRC1 is recruited to chromatin by the direct interaction between H2AK119ub1 and RYBP.

From: RYBP/YAF2-PRC1 complexes and histone H1-dependent chromatin compaction mediate propagation of H2AK119ub1 during cell division

Extended Data Fig. 1

a, Representative 15% SDS-PAGE of H2A and H2AK119ub1 octamers out of 3 independent experiments. b, Representative 1.2% agarose gel electrophoresis of H2A- and H2AK119ub1- mononucleosomes out of 3 independent experiments. 187 bp 601 DNA is labelled by biotin for capturing by streptavidin-beads. c, ChIP-qPCR analysis of RING1B, H2AK119ub1 and RYBP levels at selected gene loci in Ring1b wild type (-4-OHT) and knockout (+4-OHT) cells (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. d, Representative immunoblot of bindings of RYBP and RYBPTF/AA to RING1B/GST-BMI1 complex by GST pull-down assay out of 3 independent experiments. e, Representative coomassie-stained gels of purified recombinant proteins and protein complexes out of 3 independent experiments. Unprocessed blots and statistical source data are available in Source Data.

Source data

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