Extended Data Fig. 2: Piwil1 knockdown inhibits the proliferation and migration of PDAC cells.
a-h, Piwil1 knockdown in BxPC-3 cells reduced cell proliferation (a, n=4 independent experiments), anchorage-independent growth (b, n=3 independent experiments), xenograft tumor growth in nude mice (c, n=3 animals), cell migration (d, n=3 independent experiments), invasion (e, n=3 independent experiments), and metastatic colonization in NOD/SCID mice through either orthotopic (f, n=3 animals), spleen (g, n=3 animals) or tail vein injection (h, n=3 animals). i–l, Piwil1 knockdown in AsPC-1 cells reduced cell proliferation (i, n=4 independent experiments), anchorage-independent growth (j, n=3 independent experiments), cell migration (k, n=3 independent experiments) and invasion (l, n=3 independent experiments). BxPC-3 and AsPC-1 cells were respectively transfected with Piwil1 siRNA (a, b, d, e and i–l) or infected with shPiwil1 pseudovirus (c and f–h), and the assays were performed at 24 h post-transfection. a and i, Cell proliferation by MTT assay. b and j, Soft agar colony formation assay, with the relative number of soft agar foci per field. c, Xenograft assay in nude mice, with the time course of xenograft tumour growth. d and k, Wound healing assay, with the quantitative results of wound closure. e and l, Transwell invasion assay, with the invasive cell numbers per field at 24 h after the cells plated. f, Orthotopic xenograft assay in NOD/SCID mice. Left, the average volumes of xenograft tumors in the pancreata; right, quantification of liver metastases area. g and h, Liver or lung metastatic colonization assays in NOD/SCID mice through spleen (g) or tail vein injection (h), respectively, with the bioluminescence quantification of metastasis tumors. The mean ± SD was plotted in all graphs. Statistical analysis was performed using two-tailed Student t test. Statistical source data are provided in Statistical Source Data Extended Data Fig. 2.
