Extended Data Fig. 3: Ectopic expression of Piwil1 promotes the proliferation and migration of PDAC cells.
Ectopic expression of Piwil1 in PANC-1 cells potently promoted cell proliferation (a, n=4 independent experiments), anchorage-independent growth (b, n=3 independent experiments), xenograft tumor growth in nude mice (c, n=3 animals), cell migration (d, n=3 independent experiments), invasion (e, n=3 independent experiments), and metastatic colonization in NOD/SCID mice through either orthotopic (f, n=3 animals), spleen (g, n=3 animals) or tail vein injection (h, n=3 animals). PANC-1 cells were respectively transfected with Flag-PIWIL1 or control vector p3×Flag, and the assays were performed at 24 h post-transfection. The procedures are similar to that described in Extended Data Fig. 2. The mean ± SD was plotted in all graphs. Statistical analysis was performed using two-tailed Student t test. Statistical source data are provided in Statistical Source Data Extended Data Fig. 3.
