Extended Data Fig. 6: Downregulation of IDH3B, ACTL6A or ARPC3 by PIWIL1 is not involved in its oncogenic function in PDAC cells.
a–d, Ectopic expression of IDH3B, ACTL6A or ARPC3 barely affected cell proliferation (a, n=4 independent experiments), anchorage-independent growth (b, n=3 independent experiments), cell migration (c, n=3 independent experiments) and invasion (d, n=3 independent experiments) in BxPC-3 cells. The cells were respectively transfected with Myc-tagged IDH3B, ACTL6A, ARPC3 or control vector pCMV-Myc, and the assays were performed at 24 h post-transfection. e–h, Co-transfection of Myc-tagged IDH3B, ACTL6A or ARPC3 vectors barely affected the stimulatory effect of PIWIL1 on cell proliferation (e, n=4 independent experiments), anchorage-independent growth (f, n=3: independent experiments), cell migration (c, n=3 independent experiments) and invasion (d, n=3 independent experiments) in PANC-1 cells. The cells were respectively co-transfected with Flag-PIWIL1 and Myc-tagged IDH3B, ACTL6A, or ARPC3, with pCMV-Myc and Myc-Pinin respectively serving as negative and positive controls, and the assays were performed at 24 h post-transfection. The procedures are similar to that described in Extended Data Fig. 2. The mean ± SD was plotted in all graphs. Statistical analysis was performed using two-tailed Student t test. Statistical source data are provided in Statistical Source Data Extended Data Fig. 6.
