Extended Data Fig. 8: Pinin inhibits cell migration and metastasis but not cell proliferation and tumor growth in PDAC cells.
Pinin knockdown in PANC-1 cells barely altered cell proliferation (a, n=3 independent experiments), anchorage-independent growth (b, n=3 independent experiments), and xenograft tumor growth in nude mice (c, n=3 animals), but promoted cell migration (d, n=3 independent experiments), invasion (e, n=3 independent experiments), and metastatic colonization in NOD/SCID mice through either orthotopic (f, n=3 animals), spleen (g, n=3 animals) or tail vein injection (h, n=3 animals). The cells were respectively transfected with Pinin siRNA (Pinin-siR1 or Pinin-siR2) or scrambled siRNA (scr siR) (a, b, d, and e), or shPinin or control pSilencer (c and f–h), and the assays were performed at 24 h post-transfection. The procedures are similar to that described in Extended Data Fig. 2. The mean ± SD was plotted in all graphs. Statistical analysis was performed using two-tailed Student t test. Statistical source data are provided in Statistical Source Data Extended Data Fig. 8.
