Extended Data Fig. 6: DDX21 relocalizes from the nucleolus to the nucleoplasm upon nucleotide depletion.
From: RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells
(a, d) DDX21 and TCOF1 immunofluorescence staining in A375 melanoma cells. Experiment was repeated independently for 4 times with similar results. Scale bar represents 100μm. (b, e) Quantification of nucleoli to nucleoplasm ratio (n = 5 sections per condition, Two-sided Wilcoxon-Mann-Whitney test). Box plots represent median value and 25th and 75th percentiles. Whiskers are minima and maxima. Red asterisks indicate outliers. (c) Western blot analysis for DDX21 in A375 cells treated for 24 hours with DMSO, A77 1726 or A77 1726 plus nucleotides. ACTIN was used as loading control. Immunoblot are representative of at least 2 independent experiments. Source data are available online.
