Extended Data Fig. 4: Progesterone receptor expression and perturbation effects.
From: RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells
(a) RT-PCR for pgr using two primer sets to reveal receptor expression during early development (1 experiment). (b) GFP positive embryos were injected with pgr:flag:T2A:gfp mRNA or mismatched pgr mRNA with a pgr morpholine (MO) to reveal MO specificity. Scale bars represent 200μm. (c) In situ hybridization for crestin at 24 h.p.f. in control and pgr mRNA injected embryos with or without leflunomide treatment. Number of embryos displaying the phenotype represented is indicated. Scale bars represent 200 μm.
