Extended Data Fig. 6: LSM-8 ablation does not alter transcription termination accuracy, strand specificity nor splicing.
From: LSM2-8 and XRN-2 contribute to the silencing of H3K27me3-marked genes through targeted RNA decay
a, UCSC genome browser view showing wiggle tracks from positive (+) or negative (-) strands show the differential expression of the col-2 gene, which is upregulated in lsm-8-/- compared to WT (y axis in log2). Data shown are derived from the two independent biological RNA-seq replicas. The expression level of the neighboring genes is not affected and termination defects are not observed. All introns were as efficiently spliced in lsm-8-/- as in WT. b, G browse view showing the ModEncode ChIP-seq tracks for H3K27me1, H3K27me3 (two different antibodies) and H3K27Ac at the same genomic locus (IV:10,082,495..10, 087, 496) around the col-2 gene, as shown in (a). The col-2 gene is upregulated in lsm-8-/- compared to WT and enriched for H3K27me3, as 95% of the genes upregulated in lsm-8-/-. Statistical source data are provided in Source Data Extended Data Fig. 6.
