Extended Data Fig. 2: The tumoricidal activity of mitoxantrone depends on the NLRP3 inflammasome.
From: Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity
a, Immunoblot analysis of NLRP3 expression in purified tumour-infiltrating macrophages (TIMs). n = 2 biologically independent experiments. b, Immunoblot analysis of NLRP3 expression in purified peritoneal macrophages. n = 3 biologically independent experiments. c-f, 5 × 105 MCA205 cells were implanted sub-cutaneously into Nlrp3+/+ or Nlrp3−/− mice. When the size of tumor reached 20-45 mm2, Mitoxantrone were injected intraperitoneally. Survival analysis of Nlrp3+/+ and Nlrp3−/− mice at various times (horizontal axis) after tumor cells injection (c; n = 7 biologically independent animals). Images of tumors at day 21 after tumor cells implanted (d; n = 8 biologically independent animals). Tumor size at various times (horizontal axis) after tumor cells injection (e; n = 8 biologically independent animals). ELISA analysis of IFN-γ secretion of inguinal lymph node cells after 5-day chemotherapy (f; n = 7 biologically independent animals). e, f, Data are shown as mean ± s.e.m. Statistics were analyzed using two-sided log-rank (Mantel–Cox) test (c) or two-sided unpaired Student’s t-test (e, f). Source data are provided in Source Data Extended Data Fig. 2.
