Extended Data Fig. 4: Absence of Skin Immune Infiltration at 2 weeks post-NFI loss. | Nature Cell Biology

Extended Data Fig. 4: Absence of Skin Immune Infiltration at 2 weeks post-NFI loss.

From: NFI transcription factors provide chromatin access to maintain stem cell identity while preventing unintended lineage fate choices

Extended Data Fig. 4

a, Fluorescence in situ hybridization (FISH) of pan-bacterial 16S rRNA (rainbow colors) in cleared skin whole-mounts, co-labeled for DAPI (gray) at 2 weeks post gene knockout. Spot analysis of 16S-FISH signal was used to quantify cutaneous bacterial load. Representative images of two biological replicates. b, Immunofluorescence showing skin immune cells (CD45+) are not changed at 2 weeks following Nfib/Nfix knockout (mean and standard deviation are shown). K5 marks basal epithelial cells. n = 3 mice. c, Flow cytometry analysis of immune cell composition at 2 weeks post-NFI deletion. Mean and standard deviation are shown. n = 4 mice/genotype. d, mRNA expression (from RNA-seq) of immune-related genes in bulge-SCs at 2 weeks post-TAM. All scale bars = 20 μm. Bu, bulge. Inf, Infundibulum. Dashed lines, HF-dermal border. See also Source Data.

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