Extended Data Fig. 4: Comparison of BiPNT and EiPNT, KiPNT, NiPNT.
a, Numbers of Oct4-GFP positive colonies for PNT induced with BMP4 (BiPNT) or exogenous Esrrb (EiPNT), Klf2 (KiPNT) and Nr5a2 (NiPNT). Data shown are the mean of 3 technical replicates and are from 1 of 2 independent experiments. b, FACS analysis for the proportion of Oct4-GFP positive cells in a. c, Numbers of Oct4-GFP positive colonies for BiPNT, EiPNT, KiPNT and NiPNT with or without LDN193189 (100 nM) treatment. Data shown are the mean of 3 technical replicates and are from 1 of 2 independent experiments. d, Whole well fluorescent images of the colonies for c. Scale bars, 5 mm. The experiments were performed twice with similar results. Statistics source data are shown in Statistical Source Data Extended Data Fig. 4.
