Extended Data Fig. 6: Function of BMP4 during PNT.
a, The effect of different BMPs in PNT. BMP2, 10 ng/ml; BMP4, 10 ng/ml, BMP6, 10 ng/ml; BMP7, 10 ng/ml. Data shown are the mean of 3 technical replicates and are from 1 of 2 independent experiments. b, The inhibition of BMP signaling by LDN193189 can totally block the PNT. Data are mean ± s.d., n = 3 biological replicates. c, Immunoblotting for the activation of SMAD1/5/8 by BMP4. The experiments were performed three times with similar results. d, Quantification of the efficiency for the treatment of BMP4 in stage 2 medium. Data are mean ± s.d., n = 3 biological replicates. e, ChIP-seq tracks showing binding of Smad1/5 to the gene loci of Zbtb7b, Zbtb7c, Klf2 and Nr5a233 Statistics source data are shown in Statistical Source Data Extended Data Fig. 6. Unprocessed blots are shown in Unprocessed Blots Extended Data Fig. 6.
