Extended Data Fig. 7: Zbtb7a and Zbtb7b are required for PNT.
a, Representative images of Oct4-GFP positive colonies obtained with over-expression of mCherry, Zbtb7a and Zbtb7b at day8 of PNT without BMP4. Scale bar, 250 μm. b, Representative images of Oct4-GFP positive colonies generated by Zbtb7a or Zbtb7b in Oct4 and Sox2 mediated MEF reprogramming. Scale bar, 250 μm. c, Both Zbtb7a and Zbtb7b can promote Oct4 and Sox2 mediated MEF reprogramming. Data are mean ± s.d., n = 3 biological replicates. d, Western blot for ZBTB7A and ZBTB7B at day3 in Zbtb7a-/-, Zbtb7b-/- and double knockout (DKO)EpiSCs. e, Numbers of Oct4-GFP positive colonies for PNT (upper panel) and whole cell fluorescent images of the colonies (lower panel) induced with wildtype (WT), Zbtb7a-/-, Zbtb7b-/- EpiSCs and rescue of Zbtb7a or Zbtb7b knockout with exogenous Zbtb7a and Zbtb7b. Data are mean ± s.d., n = 3 biological replicates. Scale bars, 5 mm. f, Western blot for ZBTB7A and ZBTB7B at day3 in WT and DKO-ESC. g, q-PCR analysis for the expression of core pluripotent factors in WT and DKO-ESC. Data are mean ± s.d., n = 3 biological replicates. h, Representative images of WT and DKO ESCs maintained with BMP4/LIF medium for 2 passages. Scale bars, 250 μm. i, q-PCR analysis for the expression of core pluripotent genes Oct4, Sox2 and Nanog in WT and DKO ESCs maintained with BMP4/LIF medium at different passages. Data are mean ± s.d., n = 3 biological replicates. The experiments in a-b, d, f, h were performed three times with similar results. Statistics source data are shown in Statistical Source Data Extended Data Fig. 7. Unprocessed blots are shown in Unprocessed Blots Extended Data Fig. 7.
