Extended Data Fig. 1: BMP4 induces mouse primed-to-naive transition (PNT). | Nature Cell Biology

Extended Data Fig. 1: BMP4 induces mouse primed-to-naive transition (PNT).

From: BMP4 resets mouse epiblast stem cells to naive pluripotency through ZBTB7A/B-mediated chromatin remodelling

Extended Data Fig. 1: BMP4 induces mouse primed-to-naive transition (PNT).

a, q-PCR analysis for the expression of Oct4, Sox2 and Klf4 in ESCs and EpiSCs. Data are mean ± s.d., n = 3 independent experiments. b, Representative images for EpiSCs cultured in ActivinA/bFGF, iCD1 and iCD1+BMP4 medium for 6 days. Scale bars, 250 μm. c, Colonies of passaged cultures from reset pluripotent cells (rESCs). Scale bar, 250 μm. d, q-PCR analysis for the expression of naïve and primed pluripotent markers in ESCs and rESCs. Data shown are the mean of 3 technical replicates and are from 1 of 2 independent experiments. The experiments in b-c were repeated independently three times with similar results. Statistics source data are shown in Statistical Source Data Extended Data Fig. 1.

Source data

Back to article page