Extended Data Fig. 8: Amino acids 1-10 of RAB22A-NeoF1 are responsible for its binding to SmgGDS607. | Nature Cell Biology

Extended Data Fig. 8: Amino acids 1-10 of RAB22A-NeoF1 are responsible for its binding to SmgGDS607.

From: Chromosomal translocation-derived aberrant Rab22a drives metastasis of osteosarcoma

Extended Data Fig. 8: Amino acids 1-10 of RAB22A-NeoF1 are responsible for its binding to SmgGDS607.The alternative text for this image may have been generated using AI.

a, The fragments of RAB22A-NeoF1. b, d, 293T cells co-transfected with the indicated plasmids for 48 h were subjected to the indicated assays. c, 293T cells transfected with the indicated plasmids were lysed and subjected to immunoprecipitation using streptomycin beads and then eluted with biotin. The eluates were incubated with the purified SmgGDS607-His protein and the immunoprecipitated complex using a nickel column followed by Western blotting. Vector, vector-only control. e, f, Detection of RhoA-GTP and membrane RhoA (RhoA-MEM) in the indicated stable cell lines by Western blotting. Data in b-f are representatives of n = 3 biologically independent experiments. Vector, vector-only control.

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