Extended Data Fig. 6: ROCK activation in mammary cancers induces Atf4-regulated Creld2 transcription downstream of actin stress; Related to Fig. 6. | Nature Cell Biology

Extended Data Fig. 6: ROCK activation in mammary cancers induces Atf4-regulated Creld2 transcription downstream of actin stress; Related to Fig. 6.

From: ROCK-mediated selective activation of PERK signalling causes fibroblast reprogramming and tumour progression through a CRELD2-dependent mechanism

Extended Data Fig. 6

a, Atf4, Bip and Xbp1-spliced (green), indicating induction of Perk, Atf6 and Ire1 UPR pathways respectively in PyMT tumor cells treated with tunicamycin (10 µg/ml) to induce ER stress. Counter-labels: Phalloidin (F-actin, red); DAPI (blue). Scales: 50 µm. Experiment repeated 3 times with similar results. b, mRNA levels of Creld2 in PyMT tumor cells treated with tunicamycin (10 µg/ml), mean ± SEM (n = 3/group) relative to untreated, two-sided unpaired t-test. c, Creld2 (green) in PyMT tumor cells treated with tunicamycin. Counter-labels: Phalloidin (red); DAPI (blue). Scales: 50 µm. Mean±SEM (n = 25 untreated, 26 tunicamycin), two-sided unpaired t-test. d-f, Immunofluorescence analysis of pPerk (n = 6 KD, 5 ROCK, 5 ROCK + BMS5) d, Atf4 (n = 11/group) e, and Creld2 (n = 17 KD, 16 ROCK, 16 ROCK + BMS5) f, (all green) in K-PyMT and R-PyMT tumor cells treated with BMS5. Counter-labels: Phalloidin (red); DAPI (blue). Scales: 50 µm. Mean±SEM relative to KD, one-way ANOVA tests. See also Statistics Source Data for Extended Data Fig. 6.

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