Extended Data Fig. 10: Serine starvation drives αKG-dependent demethylation in vivo.
From: Extracellular serine controls epidermal stem cell fate and tumour initiation
a, b, OGDH (a) and SDHA (b) protein knockdown efficiency in SCC cells following 24 h in culture with or without doxycycline. Western blots performed in duplicate with similar results. shRNA sequences can be found in Supplementary Table 5. c, H&E of SCCs. Asterisks denote keratin pearls, arrows denote keratohyaline granules, arrowheads denote intercellular bridges, all signs of differentiation in SCCs. Three tumors were analyzed per condition with similar results. d, Bulk H3K27me3 levels in shRenilla, shOgdh and shSdha SCCs grafted into Nude mice on control or Ser/Gly-free diet. Three tumors were analyzed per condition with similar results. Scale bar = 50 μm. Scanned images of unprocessed blots are shown in Source Data Extended Fig. 10.
