Extended Data Fig. 8: Mof-nKO microglia display an NFκB pro-inflammatory signature.
a, Experimental outline. Microglia (CD11b+, CD45medium, PDGFRβ–, PECAM1–) were isolated from Mof-nKO and Nes-CreT/+ control brains and analysed via RNA-seq. With a false discovery rate (FDR) cut-off of 0.05, 165 genes were found to be significantly upregulated and 307 genes significantly downregulated. n = 3 animals per genotype. b, GO term (Biological Processes) analyses for differentially expressed genes (FDR < 0.05) in Mof-nKO microglia versus controls. Pathways related to inflammation such as “immune system processes” and “positive regulation of inflammatory response” were significantly enriched. Data were analysed using a Fisher exact test via the DAVID platform66. n = 3 animals per genotype. c, Expression of NFκB target genes in microglia derived from Mof-nKO brains compared to Nes-CreT/+ controls. Data are presented on a log2 scale. Expression of the majority of NFκB target genes was upregulated in microglia derived from Mof-nKO brains. n = 3 animals per genotype. Statistical source data are shown in Source Data Extended Data Fig. 8.
