Extended Data Fig. 2: Expression pattern of novel alveolar epithelial cell state enriched genes in organoids and LPS or bleomycin-treated lungs in vivo. | Nature Cell Biology

Extended Data Fig. 2: Expression pattern of novel alveolar epithelial cell state enriched genes in organoids and LPS or bleomycin-treated lungs in vivo.

From: Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis

Extended Data Fig. 2

a, Schematic of bleomycin-induced lung injury in Ctgf-GFP mice. b, Immunostaining for Ctgf-GFP (green), KRT8 (red) and SFTPC (grey) in control lung (left) and bleomycin treated lungs on day-12 (right). Representative of 3 mice. Magnified single channel images are shown on the right. White line box indicates magnified region. c, Proximity ligation in situ hybridization analysis for Ctgf (grey) and Sftpc (green) on sections derived from Sftpc-tdTomato mice administered with bleomycin. Red arrows indicate Ctgf+tdTomato+Sftpc- cells. Green arrows indicate Ctgf+tdTomato+Sftpc+ cells. d, Violin plots show the expression of Krt8 and Sfn in epithelial cell populations derived from alveolar organoid (left, n = 4,573 cells) and LPS -injured lung scRNA-seq datasets (right, n = 13,204 cells). Violin plots indicate distribution of the cells. Scale bars: 30 µm.

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