Extended Data Fig. 5: Solubility of SMAD4 nonstop extension mutants.
a-b, Stably transduced BxPC3 (a) and SW620 (b) cells were lysed in 1% NP-40 buffer. The insoluble pellet fraction was further solubilized in 2X SDS PAGE sample buffer (2% SDS). Equal volumes of NP-40-soluble and -insoluble fractions were separated and probed with SMAD4 antibodies, with β-actin as loading control (n=3 biologically independent experiments). c-d, Similar solubility analysis with the indicated EYFP fusion constructs transfected into BxPC3 (c) and SW620 (d) cells. All panels: n=3 biologically independent experiments. Unprocessed blots are provided with the paper.
