Extended Data Fig. 6: Bortezomib- and mutation-mediated rescue of SMAD4 NSExt mutant expression. | Nature Cell Biology

Extended Data Fig. 6: Bortezomib- and mutation-mediated rescue of SMAD4 NSExt mutant expression.

From: A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation

Extended Data Fig. 6: Bortezomib- and mutation-mediated rescue of SMAD4 NSExt mutant expression.

a-b, SMAD4 band quantification of biological replicates for Bortezomib-mediated rescue in BxPC3 (main Fig. 4h, n=5 biologically independent experiments) and SW620 (main Fig. 4i, n=3 biologically independent experiments) cells normalized to α/β-Tubulin and relative to the DMSO-treated WT control. c-d, The lysines shown in Fig. 7f were mutated individually (K570R, K578R, K591R) or in combination (2K = K570R+K578R or 3K = K570R+K578R+K591R) and the protein levels were monitored in transiently transfected HEK293T cells. A representative result (c) with the immunoblot quantification data from three independent experiments is shown (d). All panels: mean ± SEM, ANOVA with ***p<0.001, **p<0.01, *p<0.05. Numerical source data and unprocessed blots are provided with the paper.

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