Extended Data Fig. 5: Collagen matrix confinement determines invasion mode of breast cancer cells in the presence of adhesion-perturbing anti-E-cadherin antibody.
From: Cell–cell adhesion and 3D matrix confinement determine jamming transitions in breast cancer invasion
a, Single-cell dissemination of MCF-7 cells in collagen matrix of different density in the absence or presence of blocking anti-E-cadherin mAb SHE78-7. Micrographs represent 3 independent experiments. b, Number of individualized cells under conditions in (a). Dots for 3D represent n = 11/16 (LD), 11/10 (ID), 34/34 (HD) and, for interface, n = 39/36 (LD), 22/16 (ID), 50/39 (HD) spheroids for IgG/SHE78-7, respectively, from 3 independent experiments. c, Dependence of the number of individually invading cells out of MCF-7 spheroids on the pore diameter within 3D collagen matrices with different density, with culture conditions as in (b). d, Single-cell detachment of MCF-7 cells by SHE78-7 mAb in LD collagen. Micrograph represents 3 independent experiments. e, Single-cell detachment of MCF-7 cells along collagen-ablated tracks in HD collagen. Micrograph represents 3 independent experiments. f, Number of individualized MCF-7 cells in collagen-ablated tracks. After 48 h tracks were completely filled with cells, resulting in multicellular strands irrespective of antibody condition. Data represent n = 7/18 (24 h), 7/18 (48 h) spheroids for IgG/SHE78-7, respectively pooled from 3 independent experiments. g, Combined interference using antibody SHE78-7 in MCF-7-shCDH1 cells migrating in interface assay with collagen of different density. Micrographs represent 3 independent experiments. h, Number of individualized MCF-7 cells after E-cadherin downregulation in interface assay. Data represent the means from n=20 spheroids per condition pooled from 3 independent experiments. Red line in (b, f, h), median. P values, two-tailed Mann-Whitney test. Low (LD), intermediate (ID) and high (HD) density collagen matrices are described in Supplementary Table 1. Scale bars, 100 µm (a, g); 50 µm (d, e). For statistical source data, see Source data for ED Fig. 5.
