Extended Data Fig. 1: 3D organization of normal and mammary carcinoma tissue and the geometry of interstitial tissue tracks.
From: Cell–cell adhesion and 3D matrix confinement determine jamming transitions in breast cancer invasion
a, Immunofluorescence staining of E-cadherin or pan-cytokeratin (pan-CK), vimentin and collagen IV in normal ducts, IDC and ILC lesions. Arrowheads indicate single carcinoma cells. Example images represent 3 patient samples per condition. b, 3D reconstruction of z-projection (200 μm thickness) from IDC and ILC samples with carcinoma cells oriented in the niches formed by aligned collagen fibers. Example images represent 7 (IDC) and 3 (ILC) patient samples. c, 3D rendering of collective carcinoma strands and tissue tracks in IDC (left panel), and a different region with tissue tracks formed by collagen bundles (right panel). d,Tissue structure surrounding E-cadherin positive duct in normal breast tissue. Example image represents 3 normal breast tissue patient samples. e, Inter-fiber distance of tissue tracks formed by collagen bundles with stroma cells present or absent within the tracks. Data represent n = 49 and 24, 139 and 78, and 107 and 93 cell-free or cell-filled tissue microtracks pooled from 3 (normal breast tissue), 7 (tumor-free IDC tissue) and 3 (tumor-free ILC tissue) patient samples, respectively. Median (red line) and dots representing individual tissue microtracks. P values, two-tailed Mann-Whitney test. Scale bars, 50 µm. For statistical source data, see Source data for ED Fig. 1.
