Extended Data Fig. 2: Collective invasion of breast cancer cells in 3D collagen matrix enriched with fibroblasts or fibroblast-free matrix of different density.
From: Cell–cell adhesion and 3D matrix confinement determine jamming transitions in breast cancer invasion
a, Time-dependent collective invasion of MCF-7 spheroid into LD collagen matrix. Micrographs represent 3 independent experiments. b, Collective strand length. Data represent average length of strands from n = 9 (day 1), 13 (day 3) and 13 (day 5) spheroids (3 independent experiments). c, MCF-7 cell invasion into HD collagen matrix in dependence of cocultured MRC-5 fibroblasts. Micrographs represent 3 independent experiments. d, Length of invasion strands with and without cocultured MRC-5 fibroblasts. Data without MRC-5 represent n = 15 spheroids (day 2, 4, 6) and with MRC-5, n = 13 (day 2), 31 (day 4), 39 (day 6) spheroids/condition (3 independent experiments). e, Area of collective MCF-7 sheet moving along the collagen interface of different density. Data represent n = 47 (LD), 38 (ID), 11 (HD) spheroids/condition (3 independent experiments). f, Rat-tail (RT) collagen network at 2 (LD), 4 (ID) and 6 mg/mL (HD) density, used for 3D invasion of 4T1 cells. RT, rat tail; PD, pore diameter. g, Pore diameters at different collagen densities. Data represent n = 56 (LD), 41 (ID), 26 (HD) pores/condition (3 independent experiments). h, E-cadherin localization in 4T1 cells during collective invasion in collagen with different density (48 h). Micrographs represent 3 independent experiments. i, Cell boundary/cytoplasm ratio of E-cadherin and β-catenin intensity (conditions as in h). n.d., no detached cells detectable. Data for E-cadherin represent n = 16, 19 and 18 (strands) and 21 and 9 (detached) cells for LD, ID and HD conditions, respectively, and for β-catenin 16, 19 and 18 (strands) and 16 and 6 (detached) cells for LD, ID and HD conditions, respectively (3 independent experiments). Medians (red line); dots, means from individual spheroids (b, d, e), collagen pores (g) or individual cells (i). P values, two-tailed Mann-Whitney test. Collagen matrices described in Supplementary Table 1. Scale bars, 200 µm (a, c); 50 µm (f, h). For statistical source data, see Source data for ED Fig. 2.
