Extended Data Fig. 3: Gut-associated TTCs are responsive to local signals from the damaged intestinal epithelium.
a, Examples of tracheal branching levels assigned to each of the scores used for the quantification of tracheal coverage from light-microscopy images. Scale bar: 50 µm. b, c (top panels), Quantification of tracheal branching from confocal (b) and bright-field images (c) of Sucrose or Pe treated midguts. Two-tailed, unpaired T-test (****P < 0.0001); n = number of posterior midguts, indicated in panels. b, c (bottom panels) Representative confocal (b) or bright-field images (c) from midguts as in top panels. Scale bars: 50 µm. d, Bright-field images from Sucrose or Pe treated midguts from wild-type (control) animals or upon catalase overexpression within ECs. Scale bar: 50 µm. e, f, Scoring of tracheal branching (e) and quantification of PH3+ ISCs (f) in posterior midguts as in (d). Two-way ANOVA followed by Sidak’s multiple comparisons test (****P < 0.0001); n = number of midguts quantified, indicated in panels. g, Bright-field images of posterior midgut from control animals or upon adult-specific overexpression of bax in ECs. Scale bar: 50 µm. h, i, Quantification of tracheal branching (h) and PH3+ ISCs (i) in posterior midguts as in (g). Two-tailed, unpaired T-test (****P < 0.0001); n = number of midguts, indicated in panels. Box plots represent maxima, minima and mean. Mean value is indicated on top of boxes. Otherwise, values represent mean ± s.e.m.
