Extended Data Fig. 6: Individual sources of gut-derived Bnl are redundant for TTC remodelling but required for damage-induced ISC proliferation. | Nature Cell Biology

Extended Data Fig. 6: Individual sources of gut-derived Bnl are redundant for TTC remodelling but required for damage-induced ISC proliferation.

From: Dynamic adult tracheal plasticity drives stem cell adaptation to changes in intestinal homeostasis in Drosophila

Extended Data Fig. 6

a, Bright-field images of Sucrose or Pe treated midguts from wild-type (control) animals or upon RNAi-driven btl knockdown (btl−IR) within ISCs/EBs. Scale bar: 50 µm. b, c, Quantification of tracheal branching (b) and PH3+ ISCs (c) in posterior midguts as in (a). Two-way ANOVA followed by Sidak’s multiple comparisons test (****P < 0.0001); n = number of posterior midguts, indicated in panels. d, Representative confocal images of activated MAPK (pERK) staining (red), Arm (grey) and ISCs/EBs (esg > GFP, green) in control (Sucrose) or regenerating (Pe) adult posterior midguts from wild-type (control) animals or upon RNAi-driven btl knockdown (btl−IR) within ISCs/EBs. Dotted boxes show a magnified view of the ISCs/EBs pointed by arrows. Scale bars: 50 µm (main figure); 40 µm (close up view). e, Quantification of pERK staining intensity relative to background within ISCs/EBs in posterior midguts as in (d). Two-way ANOVA followed by Sidak’s multiple comparisons test (****P < 0.0001); n = number of ISCs/EBs from 11 posterior midguts per condition, indicated in panel. Box plots represent maxima, minima and mean. Mean value is indicated on top of boxes. Otherwise, values represent mean ± s.e.m.

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