Extended Data Fig. 10: Single-molecule subunit counting of the force-bearing integrins at thin edges of Y27632 pretreated NIH 3T3 cells.
From: A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells
a, Left, a representative tension image (45-pN RSDTP) of a single living cell treated with Y27632 showing punctate fluorescent points. Right, representative intensity traces, under continuous excitation sufficient to cause bleaching of the fluorophores, from four clusters denoted by yellow arrows in the tension image. Below, histogram and gaussian fit of fluorescence intensities for the bleaching steps in the traces (n = 39 steps from cell 1). b, Additional data were performed on two fixed cells, and similar results were obtained. (n = 38 steps for cell 2; n = 36 steps for cell 3).
