Extended Data Fig. 7: Validation of DG and SEC isolation methods. | Nature Cell Biology

Extended Data Fig. 7: Validation of DG and SEC isolation methods.

From: Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker

Extended Data Fig. 7

a, Western blot of Flotillin-1 in the 12 fractions (F1-F12) of the OptiPrep gradient. Exosomes from HEK293T, MDAMB231 and PANC-1. Blots from three biological replicates are shown. b, NTA analysis shows the nanoparticle concentration in the recovered SEC fractions (F7-F25). Bar graph shows mean ± s.e.m. of nanoparticles per ml, individual data points from three biological replicates. c, Western blotting of Flotillin-1 in exosome-rich and exosome-depleted pooled SEC fractions. Blots from three biological replicates.

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