Extended Data Fig. 3: Evaluation of specificity of FACS-based beads assay using cells silenced for tetraspanins.
a, Expression of CD9, CD63 and CD81 in shRNA-silenced cells compared to shRNA control cells. Results normalized to 18 S. Bar graphs show mean ± s.e.m. of fold-change, individual data points from three biological replicates. Statistical analysis was performed on the delta CT values by two-tailed unpaired t-test, and the exact p-values are shown. Statistical significance defined as p < 0.05. b, Quantification of the mode of exosomes from NTA analyses. Bar graph shows mean ± s.e.m, individual data points refer to three biological replicates. c, Representative profiles of size distribution of exosomes form U87 cells shRNA control, shRNA CD9, shRNA CD63 and shRNA CD81, determined by NTA. d, Representative histograms show the profile of the CD9, CD63 and CD81 in comparison to isotype control-stained beads for shRNA control, shRNA CD9, shRNA CD63 and shRNA CD81 exosomes. Bar graphs show mean ± s.e.m of MFI (geometric mean of fluorescence intensity), individual data points from three biological replicates. Statistical significance was determined by two-tailed unpaired t-test, and the exact p-values are shown. Statistical significance defined as p < 0.05.
