Extended Data Fig. 8: Validation of functional endo-siRNA target sites in HEK293 cells.
From: Global profiling of RNA-binding protein target sites by LACE-seq
a, The predicted endo-siRNA target sites in the 3′ UTRs of Birc5, Cdc42, Chk1, and Ska1 based on the Ago2 LACE-seq signal. Deduced base-pairing potentials and calculated MFEs are illustrated. Ago2 LACE-seq data represent results from three independent experiments. b-c, The relative Renilla luciferase reporter assay in HEK293 cells. Negative control (Ctrl) or endo-siRNA mimics were cotransfected with WT or seed mutants. d, The relative luciferase reporter assay of wild-type (WT) and mutant (MT) Chk1 in mouse oocytes. e-g, qPCR showing that the mRNA levels of Calm1, Bub3, and Nuf2 were not changed upon treatment with endo-siRNA-specific sponges in oocytes. Two-tailed unpaired Student’s t-test was used to calculate the P-values in b-g. Data are mean ± s.e.m., n = 3 biological replicates.
