Extended Data Fig. 1: SEC14L2/zSec14l3 colocalization analyses with secretory and endolysosomal system markers.

a, HomoloGene analysis of zebrafish sec14l3 genes among different species. Putative homologs of the zebrafish sec14l3 gene in human, mouse, xenopus, yeast and C. elegans are listed according to their protein and DNA identity. b, RT-PCR of SEC14L2 and SEC14L3 expression in COS-7 cells, monkey blood, and liver tissues. ACTIN served as a control. c, Colocalization (arrows) of endogenous SEC14L2 with EGFP-ARF1 in COS-7 cells. The region enclosed by the rectangle is shown at higher magnification in the inset. Scale bars, 10 μm (left), 5 μm (right), and 1 μm (inset). d-e, Colocalization of GM130 (cis-Golgi) with endogenous SEC14L2 (d) or zSec14l3-mCherry (e) in COS-7 cells. f-g, Colocalization of β-COP (COPI vesicles) with SEC14L2 (f) or zSec14l3-mCherry (g) in COS-7 cells. h-i, Colocalization of ERIGC53 (ER-Golgi intermediate compartment) with SEC14L2 (h) or zSec14l3-mCherry (i) in COS-7 cells. j-k, Colocalization of SEC23 (COPII vesicles) with SEC14L2 (j) or zSec14l3-mCherry (k) in COS-7 cells. l-m, Endogenous SEC14L2 immunostaining of COS-7 cells overexpressing EGFP-RAB4 (l) or EGFP-RAB7 (m). Recycling marker RAB4 and late endosome marker Rab7 are shown in green. n-o, Colocalization of SEC14L2 and endosome marker EEA1 (n) or lysosome marker LAMP1 (o) in COS-7 cells. Both markers are in green. Scale bars, 10 μm and 1 μm (inset). Golgi regions are indicated by dashed circles.

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