Fig. 5: Analysis of the timing of the spermatogenesis defect in Mov10l1–/– males.
From: Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs
a, Immunofluorescence staining of Mov10l1+/+ and Mov10l1–/– testes at 0 d.p.p., 9 d.p.p., 13 d.p.p. and 21 d.p.p. to examine the presence of germ cells (marked by DDX4), undifferentiated spermatogonia (marked by ZBTB16)39 and spermatocytes (marked by SCP3)38. DNA was stained with DAPI. Several histological sections from two different animals were analysed at all stages. Aberrant DDX4 staining observed at 9 d.p.p. is indicated (asterisk) (a magnified image is shown in c). The arrowhead indicates a single ZBTB16+ spermatogonium in a Mov10l1–/– 13 d.p.p. sample. Scale bars, 50 μm. b, Quantitative analysis of germ cell distribution in sections of 9 d.p.p. seminiferous tubules. For each genotype, the indicated number of seminiferous tubules on several sections was examined for the presence of DDX4+ germ cells. There was no statistically significant association between genotype and tubules being empty or non-empty (χ2 test, P = 0.138). c, Higher magnification of the seminiferous tubule cross-sections shown in a. Mov10l1–/– germ cells are indicated (asterisk), which show deviation from the normal DDX4 staining pattern in which strong cytoplasmic staining would surround a nucleus with a minimal signal. Scale bars, 50 μm.
